Composite

Part:BBa_K3778029:Design

Designed by: Yun Tsai   Group: iGEM21_CCU_Taiwan   (2021-09-05)


T7P-RBS-6xHis-CBD-linker-LVPRFA-eGFP-T7T


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 729
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 729
    Illegal NheI site found at 1491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 729
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 729
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 729
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part contains T7 promoter, 6xHis, RBS, CBD, linker, Thrombin cleavage site for D2A21 mimic (LVPRFA), eGFP, and T7 terminator. The T7 promoter, T7 terminator, and RBS are basic components for protein synthesis. The 6xHis is applied for protein purification. The thrombin cleavage site for D2A21 minic is added at the N-terminal of GFP to simplify the examination of D2A21 release kinetic through fluorescence recording.



Source

References